BLItz Applications
BLItz™ is a personal assay system, letting you do label-free analysis right at your bench—no core lab necessary. You can easily test column fractions for presence of your protein of interest, check protein activity before starting a complex experiment, characterize proteins in cell lysates in their folded state or monitor protein expression. BLItz gives you results in a just a few seconds or minutes—not the hours it takes with gels, blots or other methods. All you need is a drop of sample!
- Determine protein presence/absence instantly
- Analyze binding kinetics at your bench
- Quantitate proteins in seconds
- Develop immunoassays in minutes
No labels needed. When you don’t have to use labeling reagents, you save time and cut costs. Fewer assay steps translates to faster assay development, and results are more reliable when analyzing unmodified proteins.
Get data in real time. Viewing binding events in real time provides instantaneous answers, and binding curve shapes provide more information on protein quality. Real-time data also lets you measure kinetic rate constants for association and dissociation and affinity.
Use a drop of sample. Because you only need 4 µL of sample, analysis that was previously limited by small volumes is possible. BLItz lets you conserve precious, difficult to obtain reagents.
Crude sample analysis. BLItz only detects the signal change as molecules bind to or dissociate from the biosensor surface, so complex solutions are easily analyzed. Proteins can be assayed directly in cell lysates, hybridoma supernatants, up to 10% DMSO and glycerol - so sample prep time is significantly reduced.
Simple operation. Anyone can learn how to use BLItz in no time, there are no complex components or protocols. Just take BLItz out of the box and its ready to go. You can start running samples in a matter of minutes with minimal effort.
Many applications. BLItz is used for a wide range of applications in research, lead generation, bioprocess development, manufacturing and clinical applications, just like our higher-throughput Octet® platform. So whether you are in academia or industry, BLItz can easily be your personal assay system.
| Research | Lead Generation/ Optimization | Bioprocess Development | Manufacturing/QC | Pre-clinical and Clinical/Diagnostic |
|---|---|---|---|---|
| Protein expression monitoring | Affinity measurement | Protein expression monitoring | Production monitoring | Kinetic profiling of immunotherapeutics |
| Protein analysis in cell lysates | Affinity ranking | Cell line development | Contaminant detection | Anti-drug antibody testing |
| Mechanism of action | Epitope binning and mapping | Process conditions optimization | Biocomparability for lot release | Vaccine efficacy testing |
| Protein complexes in signaling | Kinetic analysis of hybridomas | Biological activity assessment | Stability testing | |
| Protein-protein interactions | Kinetic analysis of phage | |||
| Protein-DNA interactions | Protein engineering studies | |||
| Systems Biology |
Click here to learn more about other ForteBio products, including the Octet platform.
