Assay Development: Smarter
Immunoassay development can take days or even months. BLItz™ lets you see the effects of changing assay conditions on binding interactions in real time, so you can develop assays in minutes. Quickly find the best antibody pair, identify optimal conditions for a specific parameter or build and optimize an assay step by step for use on other platforms.
Comparing secondary antibodies for sandwich formation on Streptavidin biosensors. Capture antibody used was biotinylated Mouse monoclonal anti-IL-5. Rat anti-IL-5 MAb and Goat anti-IL-5 PAb were tested for sandwich forming ability to IL-5 antigen. Rat MAb formed a sandwich while Goat PAb did not.
The Advanced Kinetics mode in BLItz Pro software allows you to set up customized binding assays on BLItz.
Applications include:
- Binding pair optimization. Quickly compare antibodies for binding strength and signal. The real‑time quantitative binding information provided by BLItz lets you quickly develop the best antibody pair for your antigen, a process that often takes days using ELISA or other immunoassay platforms.
- Immunoassay development. Gain more insight into how each change in condition affects the binding of immunoassay partners. BLItz allows you to develop assays reliably, and quickly optimize a variety of experimental factors that can take months to determine via ELISA.
