Binding Kinetics: Effortless

Even though BLItz™ is small in both size and price, the label-free kinetic data it delivers is exceptional—so there is no compromise. BLItz gives you rate and affinity constants for binding interactions (ka, kd, KD) in minutes. Simple workflows using a drop of sample and a microfluidics-free design let you run kinetic assays at your personal lab bench with ease.

Dose response curve for binding of mouse IgG to Protein A
biosensors. Measured kinetic constants are reported:
(ka: 1.02E+05 (1/Ms); kd: 2.26E-04 (1/s); KD: 2.22 nM).

Extensive testing has shown that highly reliable kinetic rate constants can be measured using Dip and Read™ assays in a 4 µL drop of sample. Baseline and dissociation are performed in 250 µL of buffer in a tube, enabling highly simplified methods for measuring binding kinetics. The Basic Kinetics mode in BLItz Pro software is used to analyze the kinetic binding profile of a protein in solution interacting with a pre-immobilized protein on the biosensor. The Advanced Kinetics mode allows you load a protein on the biosensor first, then measure the kinetics of its binding to its partner.

Applications include:

  • Protein-protein interactions.  Measure affinity and rate constants of protein interactions. This information is critical to various applications such as determining mechanism of action and systems biology in early research.
  • Protein quality through binding assays.  Test the quality of expressed proteins in crude lysates by checking the binding profile against an antigen. This eliminates the significant time and effort involved in purifying the expressed protein prior to analysis with traditional methods.
  • Biotherapeutic drug development.  Perform a number of applications that involve various types of qualitative and quantitative kinetic measurements such as affinity ranking, epitope binning and mapping and studying the effect of engineering on protein drug efficacy.
  • Manufacturing/QC.  Measure kinetic binding profiles to probe the quality of manufactured protein drugs and vaccines, including lot-to-lot testing.