Label-Free System Comparison Guide

Pall ForteBio offers a number of label-free systems based on Bio-Layer interferometry (BLI) technology that meet a wide range of application needs. The table below shows a sampling of assays that can be run on each system.

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  BLItz System Octet QKe
System
Octet QK384
System
Octet RED96
System
Octet RED384
System
  Single–sample protein
detection
Automated
large molecule
characterization
High throughput
large molecule
characterization
Automated small and large
molecule
characterization
High throughput small and
large molecule
characterization
Ideal Uses
Peptide Assays  
Small Molecule Assays  
Protein Interactions (Kinetics)
VLP Assays  
Measuring Weak Binding Affinities
Measuring Tight Binding
Affinities
Multi-step Quantitation
Assays
Low-volume Sample
Measurements
At–line Bioprocess Monitoring
Quantitation in Media and Lysates
Determining Antibody Titer
Immunoassay Development
and QC
Performance
Affinity Range 1 µM – 0.1 nM 0.1 mM – 10 pM 0.1 mM – 10 pM 1 mM – 10 pm 1 mM – 10 pM
Molecular Weight >10,000 Da >5,000 Da >5,000 Da >150 Da >150 Da
Minimum Sample Volume 4 µL 100 µL 40 µL* 200 µL 40 µL*
Acquisition Rate 5 Hz 0.3, 0.6 Hz 0.3, 0.6 Hz 2, 5, 10 Hz 2, 5, 10 Hz
Simultaneous Reads 1 8 16 8 16
Specifications
Number of Spectrometers 1 1 2 8 16
Temperature Control No Ambient +4°C –
40°C
Ambient +4°C –
40°C
Ambient +4°C –
40°C
Ambient +4°C –
40°C
Microplate Positions NA 1 (96-well) 2 (96- or
348-well)
1 (96-well) 2 (96- or
348-well)
Integration with Automation No No Yes No Yes
Dimensions
Size H x W x D (cm) 17.4x15.3x22 47x43x53 77x80x80 47x43x53 77x80x80
Weight (kg) 3.3 23 68.2 24.5 68.2
* in a 384-well tilted-bottom microplate (ForteBio, part no.18-5080)
in a 96-well half-area microplate (VWR, part no. 82050-044)