Support OptionsBLItz libraryFAQsEmail Support
Customer Posters and PresentationsPosters and Presentations by Pall ForteBioTechnical NotesProduct Package InsertsInteractions NewsletterProduct DatasheetsWebinarsApplication NotesBrochuresUser GuidesSoftware Updates
Kinetic ScreeningAntibody QuantitationKinetic CharacterizationBinding KineticsCustom QuantitationAmine Reactive Biosensors: QuantitationAssay DevelopmentProtein PresenceProtein Quantitation
The BLItz system brilliantly packs the power of Dip and Read™ label-free analysis into a personal assay system. Small enough to fit on the palm of your hand (about the size of this page) and ready to go right out of the box, it can go anywhere you do. The BLItz system is also cleverly priced so everyone can have their own.
In this application note, we’ll describe best practices for getting started with the BLItz® system, as well as developing and running quantitation assays, sample and biosensor preparation, and data analysis.
This application note describes the use of the BLItz system to both streamline workflows and obtain rapid, direct quantitation of proteins in crude matrices.
This application note describes the use of the BLItz system for instant, specific protein detection in crude matrices, enabling access to realtime sample information during bioprocess development and production.
Customer Posters and Presentations
Mark Fisher, Ph.D., University of Kansas Medical Center, speaker presentation from Cambridge user meeting, May 17, 2016
Bio-Layer Interferometry offers users the ability to obtain quantitative and kinetic data for following the assembly of large protein complex systems. In this presentation, we expand the uses of this system by demonstrating that we can easily identify protein complex components attached to the BLI biosensor surface using Mass spectroscopy. From a structural standpoint we demonstrate that easily reversible complexes attached to BLI biosensor surfaces can also be deposited onto electron microscopy glow discharged copper grids and visualized using negative stain electron microscopy.
Paul Rogers, Imperial CollegePresented at the April 2013 ForteBio User Meeting, London, UKInvestigations into the design of an effective vaccine regimen to stimulate protective immunity to HIV have been ongoing for the last 30 years.
In order for a vaccine to successfully prevent infection following exposure to HIV it will likely need to generate effective antigen-specific antibodies in both plasma and the mucosa. These antibodies should be directed against conserved viral antigens, have high affinity and will preferably be neutralising, that is preventing viral infection of target cells.
During vaccine experiments, responses including the titre, affinity and class of antibodies produced in experimental models are evaluated over time to evaluate the effectiveness of the regimen. As a grant-funded academic group, involved in preclinical and clinical trials, a compact, robust, adaptable and cost-effective system to investigate the interaction of vaccine-elicited antibodies with antigens and with cell-surface receptors is highly desirable. Here we present on the use of the ForteBio's BLItz system and how it has been employed during these investigations.
Our experiences of using different biosensor surface chemistries in two separate projects are presented; namely the analysis of whole serum binding to immobilised antigen following DNA vaccination and the association of antibody-bound HIV virions with immobilised cell-surface receptors.
The ability to provide quick checks at-line for titer provides valuable insight into process variability and opportunities for increased control over them. This technical note demonstrates the utility of the BLItz system in evaluating monoclonal antibody recovery post-filtration
This technical note provides guidelines for biotinylating a protein of interest for use with Streptavidin biosensors.
Posters and Presentations by Pall ForteBio
The BLItz system enables rapid identification, quantitation and characterization of expressed protein from as little as 4 microliters of sample. Data is presented in the poster to illustrate the ease of incorporating BLI into a laboratory workflow.
The BLItz system is enabling label-free detection and analysis across protein manufacturing, purification, and processing workflows. To support customer requirements in regulated environments, Pall ForteBio offers additional tools to qualify BLItz systems and to protect the integrity of acquired data.