Protein Quantitation: Faster
BLItz™ measures concentration of your proteins without labeling or secondary reagents—even in crude media. Accurate quantitation is obtained across a 5-log dynamic range with sensitivity down to just a few ng/mL. Results that can take several hours to generate with gels, HPLC and ELISA are ready in just a few seconds with BLItz.
A 5-log dynamic range is seen for quantitation of human IgG on Protein A biosensors. Unknown samples are quantitated by interpolation of their binding rate against a standard curve.
The dynamic range of detection varies as a function of both the capture molecule and the analyte, incubation time and shaking of the sample. For example, typical measurement limits of human IgG on Protein A biosensors for a 2 minute incubation are 500 ng/mL to 4 mg/mL. Lower limits of detection may be obtained by extending the incubation time, for example to 5 minutes.
Applications include:
- Protein expression monitoring. Measure the level of expressed protein in crude samples with no need for purification. Some application areas include cell line development and clone selection.
- Bioprocess development. Optimize media and process conditions much faster with BLItz.
- Bioprocess monitoring. Test protein concentration in a 4 µL drop of sample without purification. BLItz enables monitoring for protein production in micro bioreactors, where the total volume of cultures can be just a few hundred microliters.
- Vaccine efficacy testing. Quantitate the efficacy of vaccines, such as in measuring antibody levels in immunized patient sera.
